User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/10/22

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Let it be!!!

  • Today, I ran a gel to check that the restriction was ok and also to quantify the ligation.

Quant LRE&CBG 22OcT10.JPG

  • The lanes were as follows:

1. Ladder

2. PCR product CBG restricted with ECO RI and SPEI

3. LRE plasmid restriction with ECO RI and XBA I

  • I ligated the parts and I replated the colonies from yesterday's transformation.