User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/09/09

From OpenWetWare
Jump to: navigation, search
Igem-logo-150px.png WiFi coli: A communi-colight system Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

I gotta feeling!!!


  • After inactivating the restriction, I ran a gel to check if everything went on well. Though the gel merely shows a band, I consider ok to follow on with the ligation.



CBlucsRest.JPG

  • The lanes were as follows:


1, 5. Ladder.

2. Click Beetle Green luciferase restricted with XBA I and PST I.

3. Click Beetle Red luciferase restricted with XBA I and PST I.


  • The next reactions describe the ligations to be done for a total of 20μl.



    • 1: CBG (XBA I/PST I) with J23101 labeled as CBG + J23101 Mar and date.


-H2O -----------------------> 7μL
-Buffer ligase -----------> 4μL
-Vector (plasmid) -----> 2μL
-Insert (CBG) ---> 6μL
-Ligase --------------------> 1μL

    • 2: CBR (XBA I/PST I) with J23101 labeled as CBR + J23101 Mar and date.


-H2O -----------------------> 7μL
-Buffer ligase -----------> 4μL
-Vector (plasmid) -----> 2μL
-Insert (CBR) ---> 6μL
-Ligase --------------------> 1μL


  • I also replated 5 strains of pT7 + luciferase per transformation into kanamycin plates.