User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/09/06

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LRE


  • Today, I inoculated 3 strains of LRE with pSB1C3 and 3 with J23100 in 4mL of LB with the respective antibiotic and I left them growing for 6.5hrs at 37°C. After this time, I extracted the plasmid using the High Plasmid Purification Kit of Roche.


  • I also ran the following gel to realize that the PCR purification I did the other day was not as efficient as expected, so I will need to repeat it.


Purifchafa.JPG