PCR success!!!
- I ran a gel in the morning to check that my PCR product was there. Fortunately it was, as is shown in the gel:
- The lanes in the gel are as follows:
1. Ladder.
2. Positive control.
3. Negative control.
4. PCR 1 using Prefix and the primer for the mutation (Reverse) with diluted DNA.
5. PCR 1 using Prefix and the primer for the mutation (Reverse).
6. PCR 2 using primer for the mutation (Forward) and Suffix with diluted DNA.
7. PCR 2 using primer for the mutation (Forward) and Suffix.
8. Purified plasmid J23106.
- As the product was there and the punctual mutation technique needs a lot of DNA, I decided to repeat the PCR realized yesterday. This time I only did the PCR1 and PCR2 (3 tubes each).
- To check that everything went well, I ran another gel.
- This time the lanes were:
1. Ladder.
2. Positive control.
3. Negative control.
4. PCR 1.
5. PCR 1.
6. PCR 1.
7. PCR 2.
8. PCR 2.
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WiFi coli: A communicolight system
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