User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/04/19

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Designing primers for blue promoter


  • The biopart is: BBa_K238013 designed by KU Leuven team (iGEM 2009).



  • Forward:



5’ --> 3´: GAATTCGCGGCCGCTTCTAGAGGTTTTATATGCATTAGCACTAATTGC


Length of 48nt and a GC% of 44%.


  • Reverse:



5’ --> 3’: CTGCAGCGGCCGCTACTAGTACCTCTGTTAAAAATGTTAATCAATG


Length of 46 nt and a GC% of 43%.


  • Bold Italics indicate the prefix, while bold letters indicate the suffix.