User:Marcel Quint/Notebook/medicago/2008/04/26

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check competent cells - negative control

  • use competent cells only as negative control, dissolve in 3 ml LB and plate the following dilutions on LB kan/spec plates: 2-5-50μl + spin down, dissolve in 150μl and plate everything → check on mon

use this protocol - link still missing

result from 4/24 - empty pRedroot transformation = positive control

  • transformation of electrocompetent cells with empty pRedroot worked fine no matter which amount was plated → use 5 and 50 µl for the future (agro and ec2 electroporator programs both fine)