User:Marcel Quint/Notebook/medicago/2008/04/26

Project name

Main project page Previous entry      Next entry

check competent cells - negative control use competent cells only as negative control, dissolve in 3 ml LB and plate the following dilutions on LB kan/spec plates: 2-5-50μl + spin down, dissolve in 150μl and plate everything → check on mon use this protocol - link still missing result from 4/24 - empty pRedroot transformation = positive control transformation of electrocompetent cells with empty pRedroot worked fine no matter which amount was plated → use 5 and 50 µl for the future (agro and ec2 electroporator programs both fine)