User:Mar/Notebook/2007-6-5
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protein extract fractionation (cont)
Consulted with Bill Driscoll:
- the extract that will be prepared for ZOOMing (detergent + urea added) has to be of max 0.5-0.6 mg/mL concentration
- ZOOM recommends 1-2mg of total protein per run
- Bill suggests 2 runs of 300µL each of 0.6µg/mL extract
- originally we did 1 run of 400µL of extract in final volume of 4000µL (~650x5) after preparation for ZOOMing
- pool 4 sub-samples from new extracts to make up for 2x300 = 600µL
- we start on Monday of Jun-12