protein extract fractionation (cont)
Bill reported that first run failed with establishing proper pH gradient, prob. due to not enough ampholyte content. He added more to the second run - success, went to dialysis.
I prepared next pooled extract mix with same volumes as before.
First of today's new standard BSA dilutions is wrong, did another one, this time perfect. Also, I re-assayed bad standards according to new ones.
The 2nd pool of extracts was assayed using bad standards (815 µg/mL), and diluted accordingly to nominal 0.6mg/mL (final vol. 579µL). I recalculated concentration according to new standards and what was given to Bill had conc. of 724µg/mL in 579µL.