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protein extract fractionation (cont)

2 brains (F241fg) sectioned at 200µm on cryostat.

BrdU labeling for migration assay in reelers

2 mothers at e16 weighted as above and were inj'd respectively with

* RL94a
0.38mL-45'-0.38mL or 0.45mL-45'-0.45mL

BrdU injected to a pregnant mother as in original exp.

Pups of one to be perfused at p00, and of another at ___, and then co-immunostained for BrdU and nestin .