User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/19

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June 19, 2013

  • Absorbance and fluorescence measurements taken. Inconclusive results; new samples to be made and run again.

Procedure Prep: MHA

  • Calculations from 06/10/13
    • 0.0144g MHA in 5mL PBS to make 10mM MHA
    • Add 5mL 9.4nM, 10nm AuNPs (diluted in PBS)
    • Stir overnight

Calculations: RCF to RPM Conversion

RCF=0.00001118rN2<br.> 7700g=0.00001118(8.35cm)N2<br.> N=9081.99 RPM