User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/12

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February 12, 2013

  • Fluorescence spectra were taken of the following solutions:
  1. 3.5μM 4 strand DNA in 3.5μM ThT
  2. 3.0μM 4 strand DNA in 3.5μM ThT
  3. 2.5μM 4 strand DNA in 3.5μM ThT
  4. 2.0μM 4 strand DNA in 3.5μM ThT
  5. 1.5μM 4 strand DNA in 3.5μM ThT
  6. 3.5μM 8 strand DNA in 3.5μM ThT
  7. 3.0μM 8 strand DNA in 3.5μM ThT
  8. 2.5μM 8 strand DNA in 3.5μM ThT
  9. 2.0μM 8 strand DNA in 3.5μM ThT
  10. 1.5μM 8 strand DNA in 3.5μM ThT
  11. 3.5μM ThT (for reference)
  • Fluorescence Spectra

4 repeat DNA flouresence(ThTsubtracted).jpg

8 repeat DNA flouresence(ThTsubtracted).jpg

Flouresence intensity vs concentration DNA.jpg

  • Absorbance Spectra- Concentration Comparisons

3.5micM DNAs graph.jpg 3.0micM DNAs graph.jpg 2.5micM DNAs graph.jpg 2.0micM DNAs graph.jpg 1.5micM DNAs graph.jpg

  • Fluorescence Spectra- Concentration Comparisons

3.5micM DNAs flourescence spectra.jpg 3.0micM DNAs flourescence spectra.jpg 2.5micM DNAs flourescence spectra.jpg 2.0micM DNAs flourescence spectra.jpg 1.5micM DNAs flourescence spectra.jpg