September 26, 2014
Procedures
Solution Prep
- Au stock solution:
- 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
- Myoglobin stock solution:
- 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
Nanofiber Synthesis
Protein
|
Ratio
|
Final [Au]
|
Vol Au
|
Vol Protein
|
Vol Water
|
Myoglobin |
100:1 |
0.5 mM |
0.895 mL |
0.403 mL |
3.702 mL
|
|
|
0.25 mM |
0.448 mL |
0.201 mL |
4.351 mL
|
- Measure solutions into 10 mL glass test tubes
- Stack 4 of the same type of scaffold into each tube
- Heat at 80C for 4 hours
|