User:Mackenzie K. Kelley/Notebook/Experimental Biochemistry/2017/08/30

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Objective Make AuNP using amino acids Description Prepared amino acid solutions (using cysteine, tryptophan, L-Alanine); 50ml of stock solution with a 250uM concentration (same as BSA solution used yesterday) of each. Made AuNP using each amino acid stock solution with 60, 90, 120, 150 ratios of Au: amino acid. measured pH of each solution before overnight in the oven Measured fluorescence and UV vis of all amino acid stock solutions. Data Calculations for preparing the Amino Acid solutions (from Dom's notebook) UV vis data for Amino Acid stock solutions Tryptophan is the only solution that had peak on UV, ran fluorescence Table of pH values of Au and Amino Acid solutions before oven