User:Lu Wang/Notebook/Team Allergy/2010/06/29
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Today, we did not do work with amiRNA and focused our efforts on hpRNA. We obtained undigested V0120 with a death gene insert from Team Flavor, and we have inserts from previous PCR reactions that all need to be digested and ligated into V0120.
Bet: 58.2 ng/uL; LTP (.5): 38.6 ng/uL; GFP (.5): 43.9 ng/uL; GFP: 29.1 ng/uL; LTP: 60.4 ng/uL; Backbone: 20.2 ng/uL
note: .5 denotes multistep pcr reaction from yesterday in which .5 uL of part 3 of gfp/ltp were use in one of the giant assemblies of parts 1,2,3 w/ primers A&B
Transformed amiRNA into turbo cells and plated on LB amp plates--will check for colonies tommorow (Plated at ~ 4pm)