User:Laura Flynn/Notebook/Experimental Biological Chemistry/2011/11/01
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The purpose of this experiment is to use PCR to mutate GFP so that a cysteine residue can replace the aspartic acid residue directly after the enterokinase cleavage site on the vector. AuNP synthesis was also preformed. This time, the order that the components were being added was analyzed. It was: water, HAuCl4, and then BSA.
Protocol was followed from the previous week. Two test tubes were used:
Both were kept in an oven at approximately 70 degrees for thirty minute cycles, with 10 minute breaks at room temperature in between.
In test tube one, there was aggregation that formed dark green matter at the top of the test tube.
In test tube two, there was no aggregation or visible change.
Neither of the results were expected. This could be due to contamination of the HAuCl4 stock.
5'- TAC GAC GAT GAC GAT AAG TGT CGA TGG GGA TCC GAA TTC -3'
5'- GAA TTC GGA TCC CCA TCG ACA CTT ATC GTC ATC GTC GTA- 3'