User:Laura Flynn/Notebook/Experimental Biological Chemistry/2011/10/05
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The purpose of today's experiment was to use standard concentrations Ru(bpy)(phen) to determine the sensitivity of a fluorimeter. This was then used to determine the concentration of a stock solution of maltose binding protein (MBP).
The cells cultured on 09/27/11 also underwent DNA purification as well.
MBP Concentration Determination:
So that each following stock solution had a dilution of MBP by a factor of 10.
The final concentration of MBP in each of the stock solutions was determined to be:
This was determined using the values from the UV Vis analysis. The resulting absorbance vs. wavelength graph yielded:
Each solution (A-E) was graphed as Intensity/Concentration vs. Wavelength.
The resulting graphs of both Solution A and Solution B show that the concentration of the MBP was too high to be detected on the fluorimeter.
Each of these graphs had a maximum that was determined to be:
This graph shows the collective data of every group participating in this experiment. This graph will be updated as more groups record their information. Group 3, which is denoted by asterisks, is the group that this notebook's information is from.