User:Laura Flynn/Notebook/Experimental Biological Chemistry/2011/10/04

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Objective

The purpose of today's experiment was to use standard concentrations Ru(bpy)(phen) to determine the sensitivity of a fluorimeter.

Procedure

  • Standard solutions of Ru(bpy)(phen) were created and run though a fluorimeter. Acetonitrile was used as the solvent. The solutions ranged from 0μM to 100μM in 20μM increments.
  • A graph was created using the integrated intensity of each concentration vs. the selected wavelength range (500-700 nm). The integration was done using an Excel function.

Results

The corrected intensity of Ru(bpy)(phen) was graphed against wavelength and determined to be:

RUInt.jpg

The integration intensity of Ru(bpy)(phen) in Group 3 based on each variant concentration was determined to be:

Intensity3.jpg
IntIntensity.jpg

These corrected integrated intensity values show that at a concentration of about 60 μM, the peak levels out, indicating that Ru(bpy)(phen) cannot emit more light and is fully saturated.

Notes