User:Laura Flynn/Notebook/Experimental Biological Chemistry/2011/10/04
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The purpose of today's experiment was to use standard concentrations Ru(bpy)(phen) to determine the sensitivity of a fluorimeter.
The corrected intensity of Ru(bpy)(phen) was graphed against wavelength and determined to be:
The integration intensity of Ru(bpy)(phen) in Group 3 based on each variant concentration was determined to be:
These corrected integrated intensity values show that at a concentration of about 60 μM, the peak levels out, indicating that Ru(bpy)(phen) cannot emit more light and is fully saturated.