User:Laura Flynn/Notebook/Experimental Biological Chemistry/2011/09/07

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Objective

  • To showcase the biomineralization of gold nanoparticles in Bovine Serum Albumin (BSA) so that gold nanoparticles can be created and used for future experiments

Protocol

  • 1 mL of 0.0015 mM BSA (Bovine Serum Albumen) and 1 mL of 0.25mM of HAuCl4(Chloroauric Acid) were mixed along with a 8mL buffer of 50mM acetate (pH 5.46).
  • 1 mL of 0.0015 mM BSA (Bovine Serum Albumen) and 1 mL of 0.25mM of HAuCl4(Chloroauric Acid) were mixed along with a 8mL buffer of distilled water (pH 7).
  • Each mixture was then transferred to an individual cuvette and analyzed using a UV Vis (Spectrum 200-800).
  • Each buffer was also analyzed using a UV Vis (Spectrum 200-800)
  • Each mixture was then put into an oven at about 72 degrees Celsius and then analyzed using the UV Vis every half hour.

Calculations

Data

A1.jpg A2.jpg A3.jpg

W1.jpg W2.jpg W3.jpg

Notes

Purple fibers were visible in the solution. The rising slope indicates that there are AUNPs present in the material. Purple fibers were used at a later date for analysis.


This experiment was done as a group under the leadership of Dr. Hartings at American University.