User:Klare Lazor/Notebook/Chem-496-001/2012/03/27

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Description

  • TODAY DIALYSIS OF GOLD NANOPARTICLES IN DIFFERENT VOLUMES
  • ADD BSA CONCENTRATION TO BSA CONCENTRATION CURVE

Dialysis

  • Membrane
    • Spectra/PorBiotech Cellulose Ester (CE) Dialysis Membrane MWCO: 100,000
    • 100 kD
    • wet in 0.05% sodium azide
  • 1
    • about 1,000 ml water bath
    • 5 ml AuNPs b1
  • 2
    • 100 ml water bath
    • 3.5ml AUNP b2

Data

AUNPs before dialysis batch 1 and 2.png

Figure 1: image of absorbance of AuNPs before dialysis for batch 1 and batch 2, after dialysis uv spectra will be taken again to obtain the concentration of bsa bound to the nano particles.

Notes

This area is for any observations or conclusions that you would like to note.


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