User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/26

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Objective

  • Quantify and concentrate protein from yesterday's purification
  • Run protein gel for the purification, specifically the elution fractions

Bench work

  1. Bradford protein quantification...
  2. Protein gel (loaded by Tamra Fisher):
    • ladder
    • skip
    • Pellet of shocked cells (approximately 20μL pellet, 5μL 5x loading buffer → load 10μL)
    • Periplasm/supernatant from final centrifugation (8μL + 2μL 5x loading buffer)
    • skip
    • Fraction 1C (8μL + 2μL 5x loading buffer)
    • Fraction 4E (8μL + 2μL 5x loading buffer)
    • Fraction 4F (8μL + 2μL 5x loading buffer)
    • Fraction 4G (8μL + 2μL 5x loading buffer)
    • Fraction 4H (8μL + 2μL 5x loading buffer)
    • Fraction 5A (8μL + 2μL 5x loading buffer)

Results

Protein Gel

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