User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/19

AU CHEM-570 Lab Prep

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Objective Run DNA gel with test QuikChange from yesterday Prepare media and buffers for re-growth of cells QC with pMXB10 and more cycles Bench work Ran 1% agarose DNA gel with pTXB1 QuikChange test from yesterday Lane 1: Ladder, 10μL Lane 4: pTXB1 QC +His 8/18, 5μL sample + 1μL loading buffer Lane 7: pTXB1 QC +His Stage 1, Forward 10cycles 8/18, 5μL sample + 1μL loading buffer Lane 10: pTXB1 QC +His Stage 1, Reverse 10cycles 8/18, 5μL sample + 1μL loading buffer 2-stage QuikChange protocol with pMXB10 Used most recent dilution to 10ng/μL, 8/15/11 12 cycles in stage 1, 18 cycles in stage 2, 8 minute extension step Results Gel