Objective
Analyze PCR and ligation by gel
Bench work
- Analytical minigel (done by James Page)
- 10μL ladder
- 5μL V(His)+I ligation from yesterday
- 5μL V+I ligation from yesterday
- 5μL Kay Wang's pKK223-3 miniprep
- --
- 5μL Kay Wang's pKKMDH-I12V/R81Q miniprep
- 5μL QuikChange of V(His)+I ligation from yesterday
- 5μL QuikChange negative control from yesterday
- -14 --
Results
- No bands visible in ligation or PCR lanes. This is baffling... time to start troubleshooting
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