User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/07/13

AU CHEM-570 Lab Prep

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Objective Start making competent cells of expression strain, ER2566. We're using a new protocol containing hexamminecobalt chloride in the transformation buffer and adding DTT in the process. See Hanahan, D. (1983) JMB 166 557-580. Bench work Glycerol stocks (done by Daniel Catt) Prepared glycerol stocks with 3mL cultures from yesterday #88 pTXB1+BSA Colony 1 #89 pTXB1+BSA Colony 2 #90 pTXB1+BSA Colony 3 #91 pTXB1.His+BSA Colony 1 #92 pTXB1.His+BSA Colony 2 #93 pTXB1.His+BSA Colony 3 Competent cell prep (done by Daniel Catt) innoculate 250 mL SOB with 6 colonies from E. coli ER2566 plate from a few weeks ago → 18°C w/ shaking