User:Katherine Running/Notebook/Biology 210 at AU
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<title>Katherine Running's Laboratory Notebook- Bio 210</title>
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Invertebrates 2/11
Worm Examples/Types
Earthworm-segments tighten and extend. Slinky kind of motion, worm lengthens and then pulls end of body towards the front of the body, slowly moving forward.
Flatworm-slow, creepy, flexible. glide, cautious, look around with their head multiple times before deciding to move, like checking the road before you cross, move in direction of head.
Nematode-fast, whip-like motion, angry serpent looking thing.
Five Jar Organisms
1.Bee- Arthropoda Insectca
2. Centipede- Arthropoda Chilopoda
3. Crab-Arthropoda Malacostraca
4.Tarantula-Arthropoda Arachnida
5. Millipede- Arthropoda Diplopoda
Transect Five Invertebrates
| Organism | Length in mm | Number in sample | Description |
|---|---|---|---|
| Top Niche-Arthropoda Insecta Hymenoptera | 2.5 millimeters | 1 | <img src="https://s-media-cache-ak0.pinimg.com/736x/de/4d/c6/de4dc6ba3207ec04fe70c6076349273e.jpg"onload="this.width*=0.3"> |
| Top Niche-Arthropoda Insecta Dermaptera | 5 millimeters | 1 | long antennae, 6 legs, segmented, 2 tails, long and skinny |
| Bottom Niche-Arthropoda Diplopoda | 15 millimeters | 3 | <img src="https://s-media-cache-ak0.pinimg.com/736x/52/c1/4a/52c14a81ef45becb7cf8ca38f6ae4551.jpg"onload="this.width*=0.3"> |
| Bottom Niche-Arthropoda Insecta Homoptera | 10 millimeters | 1 | <img src="https://s-media-cache-ak0.pinimg.com/736x/d5/e8/7d/d5e87d6c0c73350b79e50ad13f2f4671.jpg"onload="this.width*=0.3"> |
Plantae and Fungi 2/4
<img src="https://s-media-cache-ak0.pinimg.com/736x/93/97/4a/93974a307c4655f1bf64769cfb72252d.jpg">
| Transect Sample Plants | Location and # in Transect | Description (size and shape) | Vascularization | Specialized Structures | Mechanisms of Reproduction |
|---|---|---|---|---|---|
| #1 Clover Angiosperm | Edge of flowerbed and grass | Simple root system. don't see seeds. flower. dicot. picture's will be uploaded later. | vascularized | None | flowers |
| #2 Rose Angiosperm | Bush number 1, 8 bushes | thorny bush with flower bulbs, net-like leaves, dicot | vascularized | sexual reproduction organs | bulbs |
| #3 Grass angiosperm | Grass | flower, parallel leaves, crazy root system, monocot | vascularized | none | flowers, seeds, sexual |
| #4<img src="https://s-media-cache-ak0.pinimg.com/736x/c3/ac/21/c3ac21f336bdf86bca56e636c348a22a.jpg" style="width:250px;height:250px"> | flowerbed | thicker, waxy, red stems, crazy root system, probably flowers, monocot | vascularized | None | sexual reproduction |
| #5fern | flowerbed, fern | parallel leaves, long, spindly, narrow | vascularized | None | spores, sexual |
Microbiology and Identifying Bacteria with DNA Sequences-1/28/15
Quantifying and Observing Microorganisms
| Dilution | Agar Type | # of Colonies | Conversion | Colonies/mL |
|---|---|---|---|---|
| x10^-3 | Nutrient | 1052 | x10^3 | 1,052,000 |
| x10^-5 | Nutrient | 2231 | x10^5 | 223,100,000 |
| x10^-7 | Nutrient | 716 | x10^7 | 7,160,000,000 |
| x10^-9 | Nutrient | 425 | x10^9 | 425,000,000,000 |
| x10^-3 | TET | 241 | x10^3 | 241,000 |
| x10^-5 | TET | 48 | x10^5 | 4,800,000 |
| x10^-7 | TET | 12 | x10^7 | 120,000,000 |
| x10^-9 | TET | 38 | x10^9 | 38,000,000,000 |
Less colonies grew on the tet-plates, but they were bigger. Maybe because they had less colonies to complete with for nutrients? We had no fungi grow. Only bacteria. There were two visible types of bacterium, one we called yellow, and one we called white. However, under the microscope the white one was actually two different bacterium coexisting. Both white and yellow bacterium grew on the TET plates, but more yellow ones were able to survive than white ones.
No Archaea are expected to survive on the agar plates. Archaea live in extreme conditions.
<img src="https://s-media-cache-ak0.pinimg.com/originals/ba/b7/3a/bab73a3acdd8dba8ce7b56b2d5b74659.jpg"
onload="this.width*=0.3">
<img src="https://s-media-cache-ak0.pinimg.com/736x/ba/b7/3a/bab73a3acdd8dba8ce7b56b2d5b74659.jpg"onload="this.width*=0.3">
<img src="https://s-media-cache-ak0.pinimg.com/736x/fc/24/f9/fc24f9603c0cfb496e6a22e33e58fd62.jpg"onload="this.width*=0.3">
<img src="https://s-media-cache-ak0.pinimg.com/736x/e5/bc/ff/e5bcff34b3d61306dd410b30b0ac8822.jpg"onload="this.width*=0.3">
<img src="https://s-media-cache-ak0.pinimg.com/736x/d5/35/2a/d5352af6bd1aa11405a3316c9c5dfee2.jpg"onload="this.width*=0.3">
<img src="https://s-media-cache-ak0.pinimg.com/736x/a3/ff/3e/a3ff3e13100fa4e3033328d7e0eb8df3.jpg"onload="this.width*=0.3">
<img src="https://s-media-cache-ak0.pinimg.com/originals/6a/46/94/6a469419a9007466699e14011448fc2f.jpg"onload="this.width*=0.3">
<img src="https://s-media-cache-ak0.pinimg.com/736x/7a/23/ba/7a23ba40165c03a6a507e3e49c5cfed9.jpg"onload="this.width*=0.3">
| Colony Label | Plate Type | Colony Description | Cell Description | Gram +/- |
|---|---|---|---|---|
| NY | Nutrient | punctiform, flat, small diameter | <img src="https://s-media-cache-ak0.pinimg.com/736x/2d/dc/cb/2ddccb92afe7ce68b344ca774c8418df.jpg"onload="this.width*=0.3" > | + |
| NW | Nutrient | punction, flat, larger, squiggly edges | <img src="https://s-media-cache-ak0.pinimg.com/originals/15/67/c8/1567c8b273b6e21ce016578a6d5b336c.jpg"onload="this.width*=0.3"> | - |
| TY | TET | circular, umbinate | <img src="https://s-media-cache-ak0.pinimg.com/736x/df/a0/58/dfa058c9fb59e8a77590aacdbf9dda27.jpg"onload="this.width*=.3"> | +&- |
| TW | TET | circular, convex | <img src="https://s-media-cache-ak0.pinimg.com/originals/9e/29/3f/9e293f01087b28597b626013d4ce4911.jpg"onload="this.width*=.3"> | +*- |
Hay Infusion Culture Observations
Gritty, slimy, watery, smelly, stronger manure smell, bugs flying around, water level lower. Likely things are decomposing.
Hay Infusion-Transect 5, week 2, picture 1
<img src="https://s-media-cache-ak0.pinimg.com/originals/64/36/15/643615f9642eff30b1f54ea82f2ca5a1.jpg" style="width:518px;height:388px">
Hay Infusion-Transect 5, week 2, picture 2
<img src="https://s-media-cache-ak0.pinimg.com/736x/52/fc/35/52fc352a59f0b41b9a971803c29382c1.jpg">
Hay Infusion-Transect 5, week 2, picture 3
<img src="https://s-media-cache-ak0.pinimg.com/736x/39/5f/ef/395fefe97b671745bcbda94845c371de.jpg">
Identifying Algae and Protists-1/21/15
Hay Infusion Culture Observations
Soil settled to bottom of jar. Some leaves and grass still floating. Smells like manure. Film on top of the water.
Good observations of the hay infusion and awesome documentation for the niche 1 protists. I would have liked to see photo documentation for the second niche protists as well. Very complete lab notebook so far... great job! Make sure you date every entry so that as the notebook gets longer, things don't get confused as to which week they are from.
Hay Infusion-Transect 5, week 1, picture 1
<img src="http://media-cache-ak0.pinimg.com/236x/09/a1/2e/09a12ea4aa32ac82991b383f1a9a1e7f.jpg">
Hay Infusion-Transect 5, week 1, picture 2
<img src="http://media-cache-ec0.pinimg.com/736x/b3/e4/82/b3e482939a2d475cda0725511978d27f.jpg">
Hay Infusion-Transect 5, week 1, picture 3
<img src="http://media-cache-ak0.pinimg.com/736x/55/f8/67/55f86781baf34d64c83e67a07fd71535.jpg">
Hay Infusion-Transect 5, week 1, picture 4
<img src="http://media-cache-ec0.pinimg.com/236x/32/44/88/32448836ba8048dd9dfbe7a1bb9eedc8.jpg">
Niche 1-Top of Hay Infusion
Organism 1- Colpidium or Paramecium Bursaria
organism zooms around very quickly. Around 37 micrometers in length. stretches and reaches to eat food. clear. No flagella visible, so likely has cilia. Body oblong/oval shape.
<img src="http://media-cache-ec0.pinimg.com/736x/74/84/86/748486ca75b819ad2258504453b1718b.jpg">
Organism 2-Gonium
Many celled colony. Green. flat. Many gonium clumped together. 500 micrometers in length.No image.
Organism 3-Unidentified
110 micrometers in length. colorless. large mouth that opens and closes, as if gulping water. only swims in direction of skinny end/end with mouth. slower than Organism 1.
<img src="http://media-cache-ak0.pinimg.com/originals/92/0f/da/920fdaad4a150217e6913d3a3c1f4e86.jpg">
<img src="http://media-cache-ec0.pinimg.com/originals/08/37/ad/0837ad7a2bc67df239aee27dc986591f.jpg">
Niche 2-Bottom of Hay Infusion
Organism 4-Spirostomum
worm like. elongated body/long and narrow. No apparent flagella, though may not have cilia. wiggles. 25 micrometers in length. no image.
Organism 5-Unidentified
round head about 2.5 micrometers in length. 6-7 micrometer long tail. swims in direction of head. head is dark blue/black colored. tail is also dark. tadpole shaped. or balloon on string. not seen eating or interacting with other organisms. No image.
Biological Life at AU-1/14/15
Thanks for including the volvocine line information in the notebook too! ML
Part 1: Green Algae Evolution
| Characteristics | Chlamydomonas | Gonium | Volvox |
|---|---|---|---|
| Number of Cells in Colony | 25 | 32 | too many |
| Colony Size (μm) | 2.5/cell, 25/colony | 50 | 180 |
| Specialization of Cells | None | A little bit of specialization | Specialized |
| Mechanism of Motility | extremely fast! flagella | don't move | don't move |
| Isogamous or Oogamous | Isogamous | Isogamous | Oogamous |
| Picture | <img src= "http://media-cache-ak0.pinimg.com/736x/52/70/21/527021b483a1bf16fb0639af9881658b.jpg"style="width:278px;height:272px"/> | <img src= "http://media-cache-ak0.pinimg.com/736x/c7/cb/f7/c7cbf7d660423a9cf61a58694da0ece8.jpg"style="width:278px;height:272px"/> | <img src= "http://media-cache-ak0.pinimg.com/736x/6b/61/f3/6b61f316578ea5979bcad87f08511754.jpg"style="width:278px;height:272px"/> |
Part 2: Observing Our Transect
Great job on your transect documentation and aerial layout. ML
Data and Observations:
Biotic: clover, grass, bush type 1, bush type 2, fern
Abiotic: woodchips, soil, snow, rocks, leaves
<img src= "http://media-cache-ak0.pinimg.com/736x/93/97/4a/93974a307c4655f1bf64769cfb72252d.jpg" />
Bush Type 1
<img src= "http://media-cache-ak0.pinimg.com/736x/0e/e3/e8/0ee3e85c0d64e216c8bc9f8dea337014.jpg"/>
Bush Type 2
<img src= "http://media-cache-ec0.pinimg.com/736x/99/d9/58/99d958ecd2c5eda6cfd84037d250156f.jpg"/>
Soil/Woodchips/Rocks/Snow
<img src= "http://media-cache-ec0.pinimg.com/236x/36/bb/3e/36bb3ee84e64602895dd37a6fdee7c49.jpg"/>
Grass
<img src= "http://media-cache-ec0.pinimg.com/236x/06/bc/e8/06bce833869f906555e7bf125f25bdc5.jpg"/>
Fern
<img src= "http://media-cache-ak0.pinimg.com/736x/4b/9b/59/4b9b5979689afe03338016f3beb16fab.jpg"/>
Corner A looking at Corner B
<img src= "http://media-cache-ak0.pinimg.com/736x/c9/a5/ff/c9a5ff8d6e08c17cedf04d8823a00c28.jpg"/>
Corner A looking at Corner D
<img src= "http://media-cache-ec0.pinimg.com/236x/46/59/b0/4659b0bd56c1f4dc12de24239366dbad.jpg"/>
Corner D looking at Corner A
<img src= "http://media-cache-ak0.pinimg.com/736x/57/97/cb/5797cbc3b03b6cef5c776d72960bfa3b.jpg"/>
Corner C looking at Corner D
<img src= "http://media-cache-ec0.pinimg.com/736x/d9/b9/44/d9b944cd8707d6b696895b3408c41401.jpg" />
My First Post
Did I do it?
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