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Karmella Haynes/Notebook/Polycomb project/2010/09/12
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09/12/10
✓ p16 induction time course: add dox to 1-day plates (9 pm)
✓ Plasmid cultures: grow 100 mL cultures for KAH160/015 and 170/015 midipreps
✓ Cell culture: split confluent cultures
✓ Cell culture: expand 158-3 (158-2 failed)
✓ Thaw: HEK Gal4-EED again; pellet in 9 mL FBS, grow in 2x FBS DMEM (last two vials)
✓ Nanostring assay: start new cultures in 10 mL plates
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