User:Karmella Haynes/Notebook/Polycomb project/2010/07/28

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07/28/10

  • ✓ KAH130 screening: check RFP expression, expand selected clones

KAH130 screening
> RFP expression in dox- / vs. dox+ well (1 day dox treatment)

  1. KAH130-4, -/+
  2. KAH130-7, -/++
  3. KAH130-8, -/++
  4. KAH130-18, -/++
  5. KAH130-19, -/++
  6. KAH130-22, -/++

--> Expand dox- samples in 2x 6-well plates (FTRx+puro maintenance medium)
--> Will use one plate for Western