User:Karmella Haynes/Notebook/Polycomb project/2010/06/22

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06/22/10

  • ✓ Cell culture: expand healthy cell lines to 10 cm dishes



Cell culture
> Failed to grow:

  • 129-4 (try 129-3, 129-8)
  • 130-2 (last vial; 130-7 only alternate line; consider re-transfection)
  • 131-1 (try 131-1, 131-6, 131-9 and/or re-transfection)
  • 133-1 (do not try again, not needed)

> Successful growth:

  • 127-4
  • 128-8.3
  • 130-7

--> Process for chromatin and dox -/+ Western

> Note: for publication, focus on the following set of lines

  1. KAH126 (huPcCD-ATF)
  2. KAH154 (huPcCD)
  3. KAH128 (flyPcCD-ATF)
  4. KAH157 (flyPcCD)
  5. KAH129 (fshPcCD-ATF)
  6. KAH156 (fshPcCD)
  7. KAH130 (hCBX8-ATF)
  8. KAH158 (hCBX8)
  9. KAH131 (flyPc-ATF)
  10. KAH159 (flyPc)
  11. KAH132 (no PcCD)