06/22/10
- ✓ Cell culture: expand healthy cell lines to 10 cm dishes
Cell culture
> Failed to grow:
- 129-4 (try 129-3, 129-8)
- 130-2 (last vial; 130-7 only alternate line; consider re-transfection)
- 131-1 (try 131-1, 131-6, 131-9 and/or re-transfection)
- 133-1 (do not try again, not needed)
> Successful growth:
--> Process for chromatin and dox -/+ Western
> Note: for publication, focus on the following set of lines
- KAH126 (huPcCD-ATF)
- KAH154 (huPcCD)
- KAH128 (flyPcCD-ATF)
- KAH157 (flyPcCD)
- KAH129 (fshPcCD-ATF)
- KAH156 (fshPcCD)
- KAH130 (hCBX8-ATF)
- KAH158 (hCBX8)
- KAH131 (flyPc-ATF)
- KAH159 (flyPc)
- KAH132 (no PcCD)
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