User:Karmella Haynes/Notebook/Polycomb project/2009/12/02

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12/02/09

  • ✓ Luciferase/ RFP assay: transfections from 12/01

Luciferase/ RFP assay
> Detach cells by pipetting up and down
> Aliquot 200 μL (3x per samples "50, 100, 200, 400 ng") to black walled/ clear bottom 96-well plate (for RFP read)
> Aliquot 100 μL (3x per samples "50, 100, 200, 400 ng") to white opaque 96-well plate (for luciferase assay)
> Results: all samples flat-lined.
> Note: previous two trials relatively successful. Hold off on further experiments. Move on to Polycomb-ATF building/ transfections