User:Karmella Haynes/Notebook/PcTF Genomics/2014/11/16

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  • Transfection: Re-plate transfected cells

Transfection: Re-plate transfected cells

  • KAH126/MV2 cells in well 1 were ~50% - 60% RFP+ (very high!)
    • Could be due to low passage number (these were passage-1 cells)
  • Re-plated cells into two plates (U2OS complete growth medium):
    • 96-well: 0.2 mL per well (~5.2 E3 cells/well)
    • 24-well: 2.0 mL per well (~21.0 E3 cells/well)
  • Tomorrow (Monday), check wells for RFP signal; if confluent re-plate high RFP samples (or wait until Tuesday)