User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/22

Pc-TF Genomics

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09/22/14 6-well plates for transductions Cultures for Transduction Plate 1: U2OS Plate 2: SK-N-SH Plate 3: K562 a (suspension) Plate 4: K562 b (suspension) Plated at ~40,000 cells per well Diluted 100% confluent 10 mL harvested cells as follows: 0.3 mL in 15 mL medium (20,000 per mL) 2 mL media per well Note: miscalculated...needed 80,000 cells per well. If transduction is repeated, fix this error. For K562... a: made a 40k cell/ 2 mL / well plate b: made a 40k cell/ 1mL / well plate - transduction protocol calls for removal of 1mL medium prior to transduction. Set up this plate to avoid having to remove the 1 mL from suspended cells