User:Karlena L. Brown/Notebook/PVOH Research/2013/03/01

PVOH Research

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OBJECTIVES Continue fluorescence detection through pressure testing analysis of hydrogel samples (new modifications & better results) Straight Pipette: Hydrogel Pressure Samples Tested 3 RECALL HYDROGEL PRESSURE TESTING PROTOCOL ON 2/20/13 & FLUORESCENCE ANALYSIS PROCEDURES ON 2/13/13 Sample Order PVOH vs. Clay Ratio PVOH Type Clay Selection Amount of Hydrogel Used (g) 1 50:50 130K 110% CEC NaMT w/ DMHXLBR 0.1006 2 50:50 130K 110% CEC NaMT w/ DMHXLBR 0.1022 3 90:10 130K Laponite 0.1031 4 50:50 146K 110% CEC Laponite w/ DMHXLBR 0.1066 5 90:10 130K 110% CEC Laponite w/ DMHXLBR 0.1083 6 90:10 130K 110% CEC NaMT w/ DMHXLBR 0.1003 7 50:50 130K 110% CEC Laponite w/ DMHXLBR 0.1036 8 50:50 146K Laponite 0.1016 9 90:10 130K 110% CEC NaMT w/ DMHXLBR 0.1011 10 90:10 146K 110% CEC NaMT w/ DMHXLBR 0.1020 11 50:50 130K NaMT 0.1024 12 50:50 130K 50% CEC NaMT w/ Bu3HdP+ 0.1015 13 50:50 146K 110% CEC NaMT w/ DMHXLBR 0.0998 14 90:10 130K 50% CEC NaMT w/ Bu3HdP+ 0.1026 Notes The pipettes that provided the best fluorescence results for each sample were both the straight plastic and 90° angle bent pipettes. All samples run maintained a composition incorporating Rhodamine 6G dye All samples on 3/01/13 were tested using the straight pipette. (See figure listed below)