User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/10/10

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October 10th, 2010

1. Make XbaI-PstI double restriction to ΔRBS + GFP E004 Pure PCR product.

  • XbaI-PstI double restriction methods:
DNA -> 5 ul
Buffer 2 -> 2 ul (10% of total volume)
BSA -> 1 ul
XbaI -> 1 ul
PstI -> 1 ul
HPLC -> 10 ul (to complete total volume of 20ul)
Incubate at 37º C overnight