User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/06/17

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June 17th, 2010

1. Run gel to verify PCR from June 16th.

PCR MinBP rTth-Taq-Platinum 17Jun2010.JPG

Lanes: 1) Ladder; 2) p30-rTth; 3) p30M-rTth; 4) C- rTth; 5) C+ rTth; 6) p30-Taq; 7) p30M-Taq; 8) C- Taq; 9) C+ Taq; 10) p30-Platinum; 11) p30M-Plat; 12) C- Plat; 13) C+ Plat; 14) VF Augusto; 15) + Augusto.

2. Strains with plasmid 17 and 18 re-plated on June 16th didn’t grow, I’ll replate them and also re-plate mutant strain in TrpR to store it as stock.