User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/04/14

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April 14th, 2010

  1. Prepare and grow strains with the required plasmids:
    17: pSB1C3
    18: pSB1T3
    19: BBa_I51020
    30: pSB4A5
    Strains were grown in LB medium with the correspondent antibiotic for each plasmid.
  2. Extract plasmids with Roche kit.
  3. Double resrtiction to extracted plasmids with EcoRI and Pst1.
    Double restriction methods:
DNA -> 5ul
Buffer 2 -> 2ul (10% of total volume)
BSA (required by PstI) -> 1ul
EcoRI -> 1ul
PstI -> 1ul
HPLC -> 10ul (to complete total volume of 20ul)
*Incubate at 37ºC overnight