User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2013/09/25
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ObjectiveTo run SDS-PAGE to observe amount of hemoglobin remaining after yesterday's digestion. This should be a complimentary measurement to our UV-Vis analysis from yesterday.
DescriptionWe will be using a Bio-Rad Mini Protean system with pre-cast Mini Protean TGX gels. The manual for this system can be found here We will be running SDS-PAGE followed by gel development with Coomassie Blue staining. Below is a short description of how we will proceed. Please refer to the manual for more detailed instructions
Data-Well 1: Hemoglobin -Well 2: Pepsin 0.5 hr -Well 3: Pepsatin 0.5 hr -Well 4: Pepsin 1 hr -Well 5: Pepsatin 1hr -Well 6: Pepsin 1.5 hr -Well 7: Pepsatin 1.5 hr -Well 8: Pepsin 2 hr -Well 9: Pepsatin 2 hr -Well 10: Pepsin overnight -Well 11: Pepsatin overnight -Well 12: Hemoglobin "Figure 1. Gel electrophoresis"
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