User:Jarle Pahr/Vectors
From OpenWetWare
Jump to navigationJump to search
Notes and links regarding vectors:
AddGene vector database: http://www.addgene.org/vector-database/
Open construct database: http://www.the-odin.com/ocondb/listings.php
| Names | Size | Selective markers | Origin of replication | Copy number | Notes | Reference |
|---|---|---|---|---|---|---|
| pDrive | 3851 | Amp,Kan | pBR322 | ? | PCR Cloning kit vector | |
| pBR322 | 4,361bp | Amp,Tet | pMB1 (ColE1 relative) | ? | http://en.wikipedia.org/wiki/PBR322 | |
| pUC18 | 2686 | Amp,LacZ | pMB1 (ColE1 relative) | ? | http://www.mun.ca/biology/scarr/Plasmid_pUC18.html | |
| pKLAC2 | 9107 | Amp,Acetamidase | Aetamidase expression allows non-antibiotic selection in K. lactis | [1],2 |
Origins of replication:
| Names | Copy number | Regulation | References |
|---|---|---|---|
| pBR322 | |||
| pMB1 | |||
| ColE1 | |||
| RK2 |
Bibliography
Classification of plasmid vectors using replication origin, selection marker and promoter as criteria: http://www.picb.ac.cn/picb-dynamic/admin/pic/Classification%20of%20plasmid%20vectors%20using%20replication%20origin,%20selection%20marker%20and%20promoter%20as%20criteria..pdf
