User:Jarle Pahr/Vectors

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Notes and links regarding vectors:


AddGene vector database: http://www.addgene.org/vector-database/

Open construct database: http://www.the-odin.com/ocondb/listings.php

Names Size Selective markers Origin of replication Copy number Notes Reference
pDrive 3851 Amp,Kan pBR322 ? PCR Cloning kit vector
pBR322 4,361bp Amp,Tet pMB1 (ColE1 relative) ? http://en.wikipedia.org/wiki/PBR322
pUC18 2686 Amp,LacZ pMB1 (ColE1 relative) ? http://www.mun.ca/biology/scarr/Plasmid_pUC18.html
pKLAC2 9107 Amp,Acetamidase Aetamidase expression allows non-antibiotic selection in K. lactis [1],2


Origins of replication:

Names Copy number Regulation References
pBR322
pMB1
ColE1
RK2


Bibliography

Classification of plasmid vectors using replication origin, selection marker and promoter as criteria: http://www.picb.ac.cn/picb-dynamic/admin/pic/Classification%20of%20plasmid%20vectors%20using%20replication%20origin,%20selection%20marker%20and%20promoter%20as%20criteria..pdf