User:Jarle Pahr/Recipes

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LB (1000 mL): 10 g tryptone 5 g yeast extract 5 g NaCl


Terrfic Broth (http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8620.full?text_only=true):

900 mL H2O 12 g tryptone 24 g yeast extract 4 mL glycerol

Autoclave and add 100 mL sterile solution 0.17 M KH2PO4, 0.72 M K2HPO4.

0.17 :M KH2PO4, 0.72 M K2HPO4:

2.31 g of KH2PO4 and 12.54 g of K2HPO4 in 90 mL of H2O. Dissolve salts, adjust volume to 100 mL and autoclave.