User:Jarle Pahr/Plasmids

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Plasmid Description Length (bp) Origin of Replication Selection markers Data Comment
pJP-1 Ligation of rrnB P1 74 bp fragment into pSB-M1g cut AgeI PciI oriV (RK2) KanR 6IE58_.fas
pJP-2 Ligation of GreA 60 bp fragment cut AgeI PciI into pSB-M1g cut AgeI PciI 6105 oriV (RK2) KanR 76IE57_.fas
pJP-3 Ligation of lacUV5 PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6093 oriV (RK2) KanR
pJP-4 Ligation of "ArgI 46 bp fragment" PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6091 oriV (RK2) KanR
pJP-5 Ligation of IraP PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6106 oriV (RK2) KanR
pJP-6 Ligation of livJ 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6106 oriV (RK2) KanR
pJP-7 Ligation of His 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6106 oriV (RK2) KanR
pJP-8 Ligation of Thr 73 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6118 oriV (RK2) KanR
pJP-9 Ligation of MazEF 60 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6105 oriV (RK2) KanR
pJP-10 Ligation of pcnB 50bp fragment PCR product cut AgeI pciI into pSB-M1g cut AgeI PciI 6095 oriV (RK2) KanR
pJP-11 Ligation of GFP-LVA PCR product cut KpnI BamHI into pSB-M1g cut KpnI BamHI 7867 oriV (R2) KanR



pSB-M1g


pUC19:

pUC118:

pUC128: http://www.ncbi.nlm.nih.gov/pubmed/2853689


pUM9


pUM66


Links

http://histmicro.yale.edu/history1.htm


Bibliography

http://publisher.medfak.ni.ac.rs/2007-html/4-broj/BACTERIAL%20PLASMIDS.pdf

http://mmbr.asm.org/content/62/2/434.full

http://mic.sgmjournals.org/content/147/4/763.full.pdf


Identification and Classification of Bacterial Plasmids: http://pubmedcentralcanada.ca/pmcc/articles/PMC373151/pdf/microrev00046-0077.pdf


Microbiol Mol Biol Rev. 1998 June; 62(2): 434–464. PMCID: PMC98921. Replication and Control of Circular Bacterial Plasmids: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC98921/

Identification of cellular objective for elucidating the physiological state of plasmid-bearing Escherichia coli using genome-scale in silico analysis.: http://www.ncbi.nlm.nih.gov/pubmed?cmd=Retrieve&list_uids=19224555


Linear plasmids

http://link.springer.com/chapter/10.1007%2F7171_2007_095


Supplies

pBR322: http://www.kptkomet.no/produkter/no_89/biologi/vnr/779616/