User:Jarle Pahr/NanoDrop

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Tips and notes on using NanoDrop for DNA measurements:


Unincorporated nucleotides and RNA absorbs at 260 nm. Low concentrations (<10 ng/uL) measured on NanoDrop may plausibly be caused entirely by impurities.

Proteins and phenolic compound absorb strongly at 280 nm.

NanoDrop may not be reliable for measuring oligomer concentrations? http://www.protocol-online.org/biology-forums/posts/41901.html

260/280 ratio should be ~ 1.8

260/230 ratio should be 1.8-2.2.