User:Jamie Nunziata/Notebook/Protease Research/2015/10/14

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Objective The purpose of this lab is to use Bradford Assay to measure AuNP fiber degradation from a solution of 100nM a-chymotrypsin protease Procedure 7 samples of AuNP fibers were spun down at 300rpm for 10 minutes.These fibers will be used for our 120min, 90min, 60min, 45min, 30min, 15min, and 10min samples. 2.44µL of 41.016µM a-chymotrypsin (in Tris) and 997.6µL of Tris buffer was added to each sample tube and a blank Eppindorf tube (no fibers), making the final solution 100nM a-chymotrypsin. To each cuvette, the following was added: 1650µL of Tris buffer 750µL of incubated 1µM sample (or blank) 600µL of diluted Bradford Assay in Tris buffer (1:3) A full lab procedure can be found in Nicole Bonan's notebook entry for today Absorbance for each cuvette was measure via UV-Vis between the emission sprectrum of 400-800nm, and the data is recorded below Data