User:James Chappell/ RBS
- Midi prep the integration vectors Pick a colony in 5ml of LB and antibiotic resistance. Take 1ml and inoculate 100ml of LB containing antibiotics.
- For midi preps leave overnight at 4oC, add 70% ethanol (500ul and 500ul) into an eppendorff. Spin full spead 13rpm for 1minute. Pour off carefully ope tube and let everaporate for 30 mins. Reusupend in 200ul of water.