User:James Chappell/ RBS

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  • Midi prep the integration vectors Pick a colony in 5ml of LB and antibiotic resistance. Take 1ml and inoculate 100ml of LB containing antibiotics.
  • For midi preps leave overnight at 4oC, add 70% ethanol (500ul and 500ul) into an eppendorff. Spin full spead 13rpm for 1minute. Pour off carefully ope tube and let everaporate for 30 mins. Reusupend in 200ul of water.