March 25, 2015
- repeat synthesis using syringe pump
- prep more XRD samples (two additional types of beads)
- prep Malachite Green samples for UV-Vis measurements
Procedure
Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992) and Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix<br.>
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PVA-Alg-NaMT
- Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
- 1 g of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
- Add alginate while stirring PVA-water solution
- ~0.1 g of NaMT should be added to this mixture where applicable
- Cool dissolved and mixed solution to 35°C
- Prepare 210 mL of citric acid, calcium chloride solution
- Add 12 g (5%) citric acid
- Add 8.4 g (4%) calcium chloride
- Heat solution to 40°C while stirring
- Add the PVA-Alg-Nanocomposite mixture to a 10 ml syringe, carefully removing all air before tightening on the syringe tip
- Place the loaded syringe on the syringe pump, positioning the needle tip over the edge of the prepared citric acid/calcium chloride solution
- Set the syringe pump and press "run." The PVA solution will be added drop-wise to the cross-linking reaction mixture.
- Allow solution to cool to room temp while stirring for another 20 min
- Stir in 24 g (10%) citric acid solution for 30 min
- Stir in 14.9 g (0.5 M) sodium sulfate solution for 10 min
- Rinse with water to remove excess reactants
- Allow beads to dry in teflon plate overnight
- Clean syringe
- Pass syringe tip over flame, solution will burst out
- Use syringe to push water through tip to get any remaining solution out
- Rinse again with water until solution runs clear
Measurements
- PVA: 1.01 g
- Alg: 0.16 g
- NaMT: 0.1 g
- citric acid: 12.22 g
- CaCl2: 8.40 g
- citric acid: 11.98 + 12.24 g
- sodium sulfate: 14.94 g
Settings
10 (9.5 actual) mL at 1.5 mL/m
Notes
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