Objective
- Malachite Green calibration curve
- redo PVA-Alg/PVA-Alg-NaMT bead synthesis, same scale, 4% CaCl, smaller beads using glass pipette
Procedure
Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.>
Modified from 1/28/2015
- Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
- 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
- Add alginate while stirring PVA-water solution
- ~0.1 g of nanocomposites should be added to this mixture where applicable
- Cool dissolved and mixed solution to 35°C
- Prepare 210 mL of saturated citric acid with 2% calcium chloride
- Add 8.4 grams (4%) of calcium chloride to 210 mL of water
- Add citric acid to saturation (32 g citric acid)
- 14.7g/100mL > 30.87 g/210 for solubility comparable to boric acid
- Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
- Stir for 2 hrs to allow solidification
- Rinse with water to remove excess reactants
Measurements
- PVA-Alg
- PVA: 1.044 g
- Alginate: 0.16 g
- CaCl: 8.213 g
- citric acid: 32.01 g
- PVA-Alg-NaMT
- PVA: 0.994 g
- Alg: 0.156 g
- NaMT: 0.103 g
- CaCl: 8.381 g
- citric acid: 33.01 g
- PVA-Alg-NaMT #2
- PVA: 1.098 g
- Alginate: 0.159 g
- NaMT: 0.125 g
- CaCl: 8.9 g
- citric acid: 32.02 g
- CaCl: 8.403 g
- citric acid: 32.04 g
Notes
- PVA-Alg does not work
- PVA-Alg-NaMT #2 made in sonicator
Next Week
- Madeleine: MG Calibration curve
- Eleni: Scale up PVA-Alg-NaMT synthesis
- James: start next nanocomposite synthesis
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