User:James C. Schwabacher/Notebook/CHEM-571/2013/10/01

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Objective

Monitor the yield and kinetics of HRP-catalyzed oxidation of luminol.

Procedure

1. UV-Vis Absorbance of reactants, catalysts, and products

  1. horseradish peroxidase (Use stock solution)
  2. luminol (use stock solution)
  3. 3-aminophthalic acid (product of reaction between luminol and H2O2 catalyzed by HRP. In order to take this measurement, react (in 1:1 ratio or with slight excess H2O2) luminol with H2O2 in presence of HRP. Allow the reaction to proceed for 5 minutes; take the spectrum)

Adapted from Dr. Hartings

Data

HRP-luminol-H2O2-UVVIS.jpg

Notes