User:Ilya/Yeast/Mating pheromone response pathway
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- Crosstalk in the pathways is eliminated by formation of pathway-specific complexes (Ste5:Ste11:Ste7:Fus3, Pbs2:Ste11:Hog1)
- In S. cerevisiae, meiosis is initiated only by diploid cells deprived of glucose and nitrogen and grown in the presence of a nonfermentable carbon source 
Pheromone processing genes
- carries out C-terminal methylation of prenylated proteins including a-factor, Ras1p, and Ras2p
- encodes the prenylcysteine-dependent carboxyl methyltransferase that mediates methylation, the final step in modification of CAAX proteins; Ste14p is also membrane associated 
- Dipeptidyl aminopeptidase A involved in maturation of a-factor
- Protease involved in proteolytic step of a-factor N-terminal processing
- RAM1, RAM2
- Required for a-factor prenylation
These genes are part of the mating machinery in yeast but not necessary part of the mating pheromone response pathway.
- Swe1 - protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity
- Ras1 - pombe homolog interacts with MAPKKK
- SAG1 (α-agglutinin) - provides tight cell-cell adhesion during mating in S. cerevisiae.
- FUS1 - protein with SH3 domain required for cell fusion during mating, located at the tip of the mating projection
- BNI1/YNL271C (Saccharomyces cerevisiae)
- Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1
- Pathway architecture overview
- Tzung KW, Williams RM, Scherer S, Federspiel N, Jones T, Hansen N, Bivolarevic V, Huizar L, Komp C, Surzycki R, Tamse R, Davis RW, and Agabian N. Genomic evidence for a complete sexual cycle in Candida albicans. Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3249-53. DOI:10.1073/pnas.061628798 |
- Ashby MN, Errada PR, Boyartchuk VL, and Rine J. Isolation and DNA sequence of the STE14 gene encoding farnesyl cysteine: carboxyl methyltransferase. Yeast. 1993 Aug;9(8):907-13. DOI:10.1002/yea.320090810 |
- Kofahl B and Klipp E. Modelling the dynamics of the yeast pheromone pathway. Yeast. 2004 Jul 30;21(10):831-50. DOI:10.1002/yea.1122 |
- Banuett F. Signalling in the yeasts: an informational cascade with links to the filamentous fungi. Microbiol Mol Biol Rev. 1998 Jun;62(2):249-74.
- Bardwell L. A walk-through of the yeast mating pheromone response pathway. Peptides. 2004 Sep;25(9):1465-76. DOI:10.1016/j.peptides.2003.10.022 |