User:Howard Boland/Notebook/Art from Synthetic Biology/2011/02/04

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Check Overnight Growth BBa_J04450 Growth was fine Miniprep BBa_J04450 Eluted in 35µl Water Double Digest BBa_J04450 E|P Eluted in 35µl Water Gel BBa_J04450 E|P 1% Agarose Gel, 04-FEBRURARY-2011-17:44 GMT Lane 1: 10µL 1kbp NEB Quick Ladder Lane 2: BLANK Lane 3: 50µL BBa_J04450 E|P (Expected 1069bp (part) and 5263bp backbone (pSB1A10)) Lane 4-8: BLANK Purified BBa_J04450 E|P Purified Second Band from top, expected 5263bp backbone Ligation BBa__katE E|S & BBa_I13504 X|P & BBa_J04450 E|P Setup a ligation containing 3µl BBa_J04450 E|P 1µl BBa_I13504 X|P 1µl BBa__katE E|S 3µl Ligation Buffer 1µl Ligase 1.5µl ddH2O Transformation of Ligation The whole ligation was transformed in an carbenicillin dish