User:Hetmann/Notebook/2006-11-20

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Notes

hetmann, im confused - found the 4 tubes and nanodropped them (theyre all above 100, yay!) but I have no idea what the colors mean...

• The tubes are color coded to match the stickers on the beakers found on Desk 1 of Room 5096.

• Yellow: 104.5
• Blue: 122.3
• Green:137.7
• Pink: 161.7

Put back in freezer. LMK what the colors are and ill do the digests required for the ligations you put on 11/18.

Digestion

Doing the digests, 8 samples ea.

J36335 /sp:

• 7.6 uL DNA (64.6)
• 1 uL 10x BSA (8.5)
• 1 uL NEBuffer 2 (8.5)
• 0.2 uL SpeI (1.7)
• 0.2 uL PstI (1.7)

B0015 /sp:

• 7.6 uL DNA
• 1 uL 10x BSA
• 1 uL NEBuffer 2
• 0.2 uL SpeI
• 0.2 uL PstI

J36832 /xp:

• 7.6 uL DNA
• 1 uL 10x BSA
• 1 uL NEBuffer 3
• 0.2 uL XbaI
• 0.2 uL PstI

J36834 /xp:

• 7.6 uL DNA
• 1 uL 10x BSA
• 1 uL NEBuffer 3
• 0.2 uL XbaI
• 0.2 uL PstI

12h@45, 20min@80. in PCR machine in small room. -ZS

labeling

relabeled all the pre-digested plasmids with new labeling convention; put new labeling convention on fridge.

Goals for 11/18

1. • Progression
     • Step 1: Midiprep
       1. B0015
       2. J04500+KaiB eg.
       3. J04500+KaiC
       4. J04500+KaiA+J04500+KaiC
     • Step 2: Digestion
       1. B0015/SP
       2. J04500+KaiB/XP (J36832)
       3. J04500+KaiC/XP (J36834)
       4. J04500+KaiA+J04500+KaiC/SP (J36335)