User:Helen L. Slucher/Notebook/CHEM 571/2013/10/30

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Objective

Analysis and synthesis of more protein-AuNP materials.

Procedure

  1. Atomic Absorption
    1. centrifuge (4mL of your) samples (use the large centrifuge upstairs) 30min at 4000rpm.
    2. make standard solutions in the same manner as the last time we performed AA. But, this time make concentrations that go up to 50ug/mL
    3. Take measurements of your standards and samples
    4. Make a graph showing concentration of Au vs Au:protein ratio
  2. UV Vis
    1. centrifuge 1mL of your sample 30min at max rpm
    2. Take measurements of your samples

Data

  • Screen Shot 2013-11-02 at 9.05.31 PM.png
  1. Series 1: 230
  2. Series 2: 250
  3. Series 3: 270
  4. Series 4: 290
  5. Series 5: 310
  6. Series 6: 330
  7. Series 7: 350
  8. Series 8: 370
  9. Series 9: 390
  10. Series 10: 410
  • Screen Shot 2013-11-02 at 9.06.00 PM.png
  1. Series 1: 30
  2. Series 2: 50
  3. Series 3: 70
  4. Series 4: 90
  5. Series 5: 110
  6. Series 6: 130
  7. Series 7: 150
  8. Series 8: 170
  9. Series 9: 190
  10. Series 10: 210