User:Helen L. Slucher/Notebook/CHEM 571/2013/08/28

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Overview Synthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is also surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. BSA-Au Nanoparticles Add 1mL of the (~2.5mM -note the exact concentration) gold (HAuCl4) solution to a 10mL volumetric flask Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA Add deionized water up to 10mL Transfer solution to a test tube and cap with aluminum foil Heat in oven at 80C for 3 hours Transfer solution to a plastic falcon tube (with blue cap) Stock solutions made Gold solution (HAuCl4·3H2O) 0.0100g in 0.0100mL water → 2.54mM BSA solution 0.0104g BSA (MW = 66776g/mol) in 0.0100mL water → 15.6μM Citrate-Au Nanoparticles Take 50mL of the HAuCl4 solution from the 250mL volumetric flask. Note the concentration Heat this solution to boiling while stirring Add 3mL 1.5mL of 1% (w/v) sodium citrate Boil solution for another 40 minutes Cool to room temperature and measure the volume Determine the final concentration of gold and citrate Stock solutions made Gold solution (HAuCl4·3H2O) 0.0245g in 0.2505mL water → 0.249mM Sodium Citrate (Na3C6H5O7·2H2O) 0.1010g in 10.0mL → 1.01% Data Citrate AuNP Peak Wavelength = 518 nm Absorbance at Peak = 0.612 Final Concentration A450=0.389 Abs (518)/ Abs (450)= .612/ .389= 1.57 A518/A450=1.57 Using the data found here: 1.57 = 12 d/nm and a molar absorptivity of 1*10^8. ε450=1.09*108 M-1cm-1 Beer's Law gives an equation for the absorption of light and the properties of the solution. [math]\displaystyle{ \ A=\epsilon bc }[/math] c=A450/ε450 c=3.57*10-9 mol L-1 References Dr. Harting's AU Biomaterials Design Lab