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Helen L. Slucher/Notebook/CHEM-572/2014/04/01
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Notebook
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CHEM-572
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2014
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04
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Goals
Make beta method GNPs with all three proteins (HRP, hemoglobin, myoglobin)
Spin down gamma method HRP pellet
Remake solutions so they are more concentrated
Notes: Kinetics
Reduction of heme group
OPD, look for diamino...change of concentration over time, rates
Change in absorbance = change in concentration
Find molar absorptivity
Deoxygen/oxygen bound
Take off clear super, spin down until it doesn't show GNP activity
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