Goals
- Make beta method GNPs with all three proteins (HRP, hemoglobin, myoglobin)
- Spin down gamma method HRP pellet
- Remake solutions so they are more concentrated
Notes: Kinetics
- Reduction of heme group
- OPD, look for diamino...change of concentration over time, rates
- Change in absorbance = change in concentration
- Find molar absorptivity
- Deoxygen/oxygen bound
- Take off clear super, spin down until it doesn't show GNP activity
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