User:Helen L. Slucher/Notebook/CHEM-572/2014/04/01

From OpenWetWare
Jump to navigationJump to search
Project name Main project page
Previous entry      Next entry


  1. Make beta method GNPs with all three proteins (HRP, hemoglobin, myoglobin)
  2. Spin down gamma method HRP pellet
  3. Remake solutions so they are more concentrated

Notes: Kinetics

  • Reduction of heme group
  • OPD, look for diamino...change of concentration over time, rates
  • Change in absorbance = change in concentration
  • Find molar absorptivity
  • Deoxygen/oxygen bound
  • Take off clear super, spin down until it doesn't show GNP activity